1. Prepare a list of genes with a header “Symbol”, in one single column. Please use HGNC/MGI symbols only.
  2. You can use in-built reference background gene set, or a custom background gene set for enrichment analysis
  3. If you opt for enrichment with custom background gene set, prepare of list of background genes, with a header “Symbol”, in one single column. Please use HGNC/MGI symbols only.
  4. In user dashboard, click on “Analyze a new experiment”
  5. Intake form loads and in intake form, select “Only my genes of interest/DE genes” under “What data would you like to analyze” drop down menu
  6. Select appropriate organism and Data type
  7. Upload the input file by clicking on “Choose file” and select file prepared in step 1
  8. Uploaded data is displayed in file content preview.
  9. Under “Gene symbol” column, select the column with genes (gene symbols) with header “Symbol”
  10. Click on “select the reference type” drop down menu
  11. If you want to use custom background, follow steps 13 to 16. If you want to use an in-built reference gene set, follow step 12.
  12. To use the in-built reference gene set, click on “all genes/proteins in the transcriptome/proteome” available under “select the reference type” drop down menu and skip steps 13-16.
  13. To use custom background, click on “a custom set of genes” available under “select the reference type” drop down menu.
  14. Click on “Choose file” available under “Upload reference file (Mandatory)” to upload custom background gene set. Upload file prepared in step 3.
  15. Uploaded data is displayed in file content preview.
  16. Under “Gene symbol” column, select the column with genes with header “Symbol”
  17. Click on upload and proceed with analysis.